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class="xafgt4t22k" id=tagContent> <div id="xafgt4t22k" class="tagContent selectTag" id=tagContent0><p><strong><strong>人食欲素/阿立新BOX-BELISA試劑盒</strong><br/>實(shí)驗(yàn)材料與試劑配制:<br/>1.儀器與材料:酶標(biāo)儀(使用前預(yù)熱30分鐘)，微量加液器、吸頭、蒸餾水或去離子水，濾紙。<br/>2.洗液的配制:按1:30的比例配制洗液備用。三，樣品收集、處理及保存1).細(xì)胞培養(yǎng)上清:<br/>適用干檢測(cè)體外培養(yǎng)的細(xì)胞分沙性成份，用無(wú)菌管收集細(xì)胞上清液，以1000xq離心15分鐘，收集上清。2) 細(xì)胞:<br/>用PBS反復(fù)洗滌細(xì)胞3次，調(diào)整細(xì)胞濃度達(dá)到104-106/ml 左右，通過(guò)反復(fù)凍融，使細(xì)胞破壞并放出細(xì)胞內(nèi)成份，或者細(xì)胞超聲粉碎，離心取上清液檢測(cè)。3)血清:<br/>在室溫下，血液自然凝固，以1000xq離心15分鐘，取上清待測(cè)，4)血漿:<br/>應(yīng)根據(jù)標(biāo)本的要求選擇EDTA或檸檬酸鈉作為抗凝劑，混合后靜置10-20分鐘后，以1000xq離心15分鐘，收集上清。5)體液:<br/>包括胸腹水、腦脊液，分泌物等，使用不含熱原和內(nèi)毒素的離心管收集，以1000xq離心15分鐘，收集上清<br/></strong><br/><strong>人食欲素/阿立新BOX-BELISA試劑盒</strong><strong><br/>人轉(zhuǎn)移因子 TF ELISA試劑盒<br/>人抗突觸前膜抗體 PsmAb ELISA試劑盒<br/>人乳鐵傳遞蛋白/乳鐵蛋白抗體IgG LF/LTFAb-Ig ELISA試劑盒<br/>人EB病毒IgG EBvIgG ELISA試劑盒</strong><br/><br/></p><p><br/></p></div> <div class="xafgt4t22k" id="nr_textbox"><link 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